Figure 7.

RAFL-1 antibody reduces microvascular density in MDA-MB-231 human breast tumors growing in the MFP of mice. Tumors from RAFL-1-treated (A) or rat IgG control antibody-treated (B) mice were removed, frozen sections were cut, and microvessels were detected by anti-CD31 antibody. Microvascular densities were determined on six individual slides per experimental group and the results were averaged. The mean number of vessels per square millimeter was 164±7 and 57±5 in control and RAFL-1-treated groups, respectively. Representative images of tumors derived from control and RAFL-1-treated mice are shown. Note the absence of microvasculature and the extent of necrosis in tumor from RAFL-1-treated mice compared to a fully viable and highly vascularized tumor from the control group.