Fig. 2.
Dose–response curves of BMP2, BMP4, BMP9, and murine IL-6. Primary hepatocytes were isolated from perfused livers of C57BL/6J mice, seeded at 1.5 × 105 cells per well, allowed to attach to the collagen-coated 12-well plates, and treated with BMP2, BMP4, BMP9, and murine IL-6 at concentrations ranging from 0.1 to 100 ng/ml for 12–16 h. Hepcidin and 18S ribosomal protein (S18 RP) (a normalization gene) expression was assayed by quantitative real-time RT-PCR using TaqMan probes. All samples were processed in triplicate, and the mean and SEM are shown.