Fig. 1.

Hoxc8-DNA interaction detected by ChIP assay. MEF cells transfected with Hoxc8 expression plasmid (pcDNA4-Hoxc8) or pcDNA4 empty vector were used for ChIP assays. E15.5 mouse embryos were also used for ChIP assay. The assays were performed by using antibody recognizing Hoxc8 or no antibody as a negative control. Immunoprecipitated DNA was analyzed by quantitative PCR using Hoxc8 binding site-specific primers. Cdh11, Ncam, opn, pedf, and Zac1 were enriched >3-fold greater than no antibody negative control. The ChIP assays were repeated in three independent experiments with similar results.