Fig. 2.

Effects of Hoxc8 on pedf, Ncam, Zac1, opn, and Cdh11 mRNA and promoter expressions in MEF and HeLa cells. (A) Quantitative RT-PCR analysis of pedf, Ncam, Zac1, opn, and Cdh11 mRNA expression in MEF cells transfected with Hoxc8 expression plasmid (pcDNA4-Hoxc8) or pcDNA4 empty vector. All results were normalized to the β-actin value. (B) Relative luciferase activities for different constructs. HeLa cells were plated at a density of five × 10⁴ cells per well in 12-well plates for transfections. pedf, Ncam, Zac1, opn, and Cdh11 promoter constructs (0.3 μg) were cotransfected with 0.1 μg of Hoxc8 expression plasmid (pcDNA4-Hoxc8) or 0.1 μg of pcDNA4 empty vector separately. Transfected cells were harvested and lysed in 48 h after transfection. The luciferase activity was measured and normalized to the Renilla luciferase level as internal controls. Data are shown with the mean of ± SD of triplicates.