Fig. 2.

Selective utilization of NHEJ and HR during development. At E14.5, apoptosis is found in the forebrain proliferative VZ of the Xrcc2^−/− embryo, whereas in the Lig4^−/− embryo, TUNEL-positive cells are predominantly located in the postmitotic SVZ (indicated by arrows). (A and B) Magnification: ×200; V, ventricle. (C and E) Doublecortin identifies scattered differentiating cells in the VZ. (D and E) DAPI staining shows the whole embryonic forebrain. (Magnification: ×200.) (F–K) TUNEL-positive cells did not colocalize with doublecortin-positive cells in Xrcc2^−/− embryos (F–H) but did so in Lig4^−/− embryos (I–K). (Magnification: ×1,000.)