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. 2003 Feb 15;31(4):e18. doi: 10.1093/nar/gng018

Figure 1.

Figure 1

Spot-localized, contaminating fluorescence, detected only in the green channel, is present on both commercial and in-house printed microarrays. (A) Corning preprinted array (CMT12015426 yeast S288C v. 1.32, lot 28300001), and (B) OpArray YC2A35 (Operon) were scanned immediately after opening their original packaging. (C) Corning GAPS I aminosilane coated glass slides and (D) ArrayIt superamine coated glass slides (TeleChem) were spotted with water, 3× SSC, 10 mM Tris, or Micro Spotting Solution (MSS) with or without 70mer oligonucleotides (oligo) resuspended to 40 µM. No spot-localized fluorescence was detected in the red channel.