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. 2003 Feb 15;31(4):e18. doi: 10.1093/nar/gng018

Figure 5.

Figure 5

Figure 5

Post-printing treatment removes some but not all spot-localized, contaminating fluorescence of in-house printed arrays. (A) Yeast 70mer oligonucleotides (Operon) were spotted in ArrayIt Micro Spotting Solution (TeleChem) on GAPS I amino-silane slides and given a post-printing treatment as described in Materials and Methods. Contaminating fluorescence was visualized and quantified after print (PR), post-printing treatment (PP) and mock-hybridization (MH). Sun symbol denotes panel where brightness and contrast were adjusted to visualize the spots. (B) Spot intensities (median pixel intensity – median background pixel intensity) for each of four arrays (arrays 1–4) were averaged and graphed with standard deviations (n = 96 per array).