Fig. 2.

Inorganic carbon (Ci)-dependent photosynthetic O₂ evolution and internal Ci accumulation in wild-type C. reinhardtii and the ad1 mutant. (A and B) Response of Ci-dependent photosynthetic O₂ evolution (measured at pH 7.3 and a chlorophyll concentration of 20 μg/ml) to NaHCO₃ concentrations in wild-type (cw10; ●), ad1 (▴), and LciB-complemented ad1 (ad-lb-p3; ■) cells acclimated to low CO₂ (A) or very low CO₂ (B). (C) acid-labile intracellular Ci accumulation by wild-type (CC125; ●) and ad1-3 (a walled progeny of ad1; ■) cells (25 μg/ml chlorophyll) acclimated in low CO₂ for 24 h. ¹⁴C-uptake was measured at pH 7.3 and an initial added Ci concentration of 50 μM (NaH¹⁴CO₃).