Fig. 3.

Cloning of Ad1 and complementation of ad1 and pmp1 by LciB. A. Integration of pSP124s resulted in a deletion of −36 kb of genomic DNA. A schematic presentation of the exon-intron structure of LciB is shown with exons of the coding region (filled boxes) and introns (black lines) indicated. (B) Southern blot analysis indicating the presence of the LciB gene in wild-type cw10 and one of the ad1 strains (ad-lb-p3) complemented with a PCR-amplified genomic DNA fragment containing the LciB gene, but not in ad1. Genomic DNA was digested with SalI, separated by agarose gel electrophoresis and hybridized against an LciB gene specific probe. (C) Northern blot analysis indicates the expression of LciB recovered in one of the complemented ad1 strains (ad-lb-p3). Total RNA (10 μg per lane) was isolated after high CO₂ grown cells (0 h) were transferred into low CO₂ for 2 and 4 h. Hybridization was performed with an LciB gene-specific probe.