Fig. 6.

Expression of genes of the LciB family under different CO₂ conditions. RNA gel-blot analysis of LciB, LciC, and LciD expression (probed with PCR fragments specifically corresponding to LciB, LciC, and LciD 3′ UTR regions, respectively) was performed in wild-type (cw10), ad1, and cia5 mutants. Total RNA (10 μg per lane) was isolated from high CO₂-grown cells or from cells acclimated to low CO₂ (air) or very low CO₂ (50 ppm) for the time durations indicated.