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. 2006 Jun 19;103(26):9952–9957. doi: 10.1073/pnas.0510528103

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© 2006 by The National Academy of Sciences of the USA

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Fig. 4. — Expression of proapoptotic genes in chk2 and p53 mutants. mRNA levels were quantified by RT-PCR of cDNA derived from total RNA isolated from imaginal discs of irradiated (4,000 R) or control (0 R) larvae. For each experiment, RT-PCRs were run in triplicate and normalized to the average of actin and α-tubulin controls, and the average and the standard deviation were calculated and normalized to the values for unirradiated WT (0 R). (A) Sample data from an experiment in which rpr levels show a statistically significant (P < 0.05) increase in WT and chk2 larvae but not p53 larvae at 4 hr after irradiation. (B) Sample data from an experiment in which hid levels show a statistically significant (P < 0.05) increase in p53 larvae but not WT or chk2 larvae at 18 hr after irradiation.