Skip to main content
. 2003 Nov;5(6):533–545. doi: 10.1016/s1476-5586(03)80037-4

Figure 1.

Figure 1

(a) Representative immunofluorescence staining of hLAMPs in tumor sections of breast carcinoma patients. The LAMP fluorescence is displayed in red; the fluorescence of the cell nuclei is displayed in green. The image on the left shows a (i) normal duct (FOV 80 x 80 m). The central image shows (ii) hyperplasia (160 x 160 m). The image on the right shows a (iii) ductal carcinoma (80 x 80 m). Histological grading of the tumor sections was verified by hematoxylin and eosin staining of neighboring sections of the same tumor. (b) Western blots probing for (i) hLAMP-1 or (ii) hLAMP-2 in cell lysates from HMECs and human breast cancer cell lines used in this study. Left to right lane: MCF-12A, MCF-7, MDA-MB-231, and MDA-MB-435 cells. hLAMP-1 and hLAMP-2 antibodies revealed immunoreactive bands between 100 and 140 kDa that are typical of mature, highly glycosylated LAMP proteins. Immunofluorescence staining of hLAMP-1/hLAMP-2 in tumor sections as well as Western blots for hLAMP-1/hLAMP-2 prove that this protein is ubiquitously expressed in breast tissues and human breast cancer cell lines with different degrees of malignancy.