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Representative immunofluorescence staining of invasive/metastatic MDA-MB-231 breast cancer cells exposed to (a) pH 7.4 (control), (b) pH 6.8, or (c) pH 6.4. Examples from three images (80 x 80 m) are shown for each pH to demonstrate that in control cells (pH 7.4), lysosomes were mainly perinuclear, whereas in cells at acidic extracellular pH (pH 6.8 and pH 6.4), lysosomes were more scattered and shifted toward the cell periphery.
