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Phase contrast photomicrographs depicting (a) cells emerging from explant after 5 days in vitro. The cells on serial passaging develop into an early passage cell line HNGC-1 (b). Most cells appear flat with small soma and large cytoplasmic extensions. A striking morphological change has occurred in the HNGC-2 cell line (c) on transformation, wherein cells on confluency pile up, lack cytoplasmic extensions, and display a small refractive cell body (c). Original magnification, x 10.
