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. 2003 Nov;5(6):520–532. doi: 10.1016/s1476-5586(03)80036-2

Figure 7.

Figure 7

Expression of Tuj1 in HNGC-1 and HNGC-2 cell lines. (A) Immunoreactivity to Tuj1 was observed only in HNGC-1 as intense filamentous staining (a) in a small population of cells. A single cell from HNGC-1 displaying the characteristic expression in the mitotic spindle is shown in (c). There was no immunodetectable expression of Tuj1 in the HNGC-2 cell line. (b) The nucleolus of the same cells stained with DAPI is represented in (d). Original magnification, x 63. (B) Expression of Tuj1 was analyzed at the RNA level by RT-PCR using Tuj1-specific primers. The RT-PCR products were analyzed on 1% agarose gel stained with ethidium bromide. Lane 1 corresponds to the molecular weight marker 1 kb. Lanes 2 and 3 show expression of the 540-bp Tuj1 in both cell lines HNGC-1 and HNGC-2. Lanes 4 and 5 represent β-actin PCR used as RNA loading control for the HNGC-1 and HNGC-2 cell lines, respectively. The RT-PCR products were confirmed by sequencing of the cDNA products.