Figure 8.
Effect of 2-ME2-induced VEGF-A in GH3 cells on neighboring endothelial cell proliferation. The effect was determined by indirect coculture using Transwell culture system. The detailed experimental procedure has been described in the Materials and Methods section. Endothelial cell proliferation was determined by measuring the radioactive thymidine incorporation using a liquid scintillation counter. Data displayed as mean±SD from three separate experiments. P value was determined by Student's t-test. *P < .05 vs untreated control; **P < .01 vs 2-ME2-treated cells.