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. 2003 Sep;5(5):427–436. doi: 10.1016/s1476-5586(03)80045-3

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Copyright © 2003 Neoplasia Press, Inc. All rights reserved

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Histone acetylation state is linked to DNA methylation state at the maspin promoter in pancreatic cells. Acetylation of histones H3 (A) and H4 (B) in the maspin and GAPDH promoters was analyzed using a quantitative chromatin immunoprecipitation assay. Gene promoter-specific real-time PCR was carried out on DNA from the immunoprecipitated chromatin as well as input DNA. A representative real-time PCR graph and the fold enrichment of histone acetylation are shown. Assessment of histone acetylation state of the ubiquitously expressed GAPDH was performed as a positive control.

Histone acetylation state is linked to DNA methylation state at the maspin promoter in pancreatic cells. Acetylation of histones H3 (A) and H4 (B) in the maspin and GAPDH promoters was analyzed using a quantitative chromatin immunoprecipitation assay. Gene promoter-specific real-time PCR was carried out on DNA from the immunoprecipitated chromatin as well as input DNA. A representative real-time PCR graph and the fold enrichment of histone acetylation are shown. Assessment of histone acetylation state of the ubiquitously expressed GAPDH was performed as a positive control.