TABLE 1.
Correlation of hemolytic phenotype with hemolysin gene content as determined by multiplex PCRa
| Organismb | Phenotype | No. of isolates with each hemolysin genotype
|
No. of isolates | ||||
|---|---|---|---|---|---|---|---|
| ahh1 (genotype 1) | asa1 (genotype 2) | ahh1 + asa1 (genotype 3) | ahh1 + A. hydrophila aerA (genotype 4) | No hemolysin gene (genotype 5) | |||
| A. hydrophila (87) | Hemolytic | 35c | 1 | 46 | 82 | ||
| Nonhemolytic | 3d | 2d | 5 | ||||
| A. veronii bv. sobria (21) | Hemolytic | 9 | 4 | 1d | 14 | ||
| Nonhemolytic | 2d | 1d | 4 | 7 | |||
| A. caviae (15) | Hemolytic | 1d | 1 | ||||
| Nonhemolytic | 3d | 11 | 14 | ||||
| A. veronii bv. veronii (1) | Hemolytic | 1 | 1 | ||||
| A. trota (2) | Hemolytic | 2 | 2 | ||||
| A. jandaei (1) | Hemolytic | 1 | 1 | ||||
| A. schubertii (1) | Nonhemolytic | 1d | 1 | ||||
| Total | 46 | 11 | 5 | 48 | 18 | 128 | |
a
All isolates produced 16S rRNA amplicons.
b
Numbers in parentheses are the total number of isolates examined within each species.
c
Includes positive control strain CA11.
d
DNA hybridization was performed with all of these isolates.