TABLE 4.
Transduction of flagellar antigen specificities
| Donor straina | Recipient strain | Counterselecting H antiserum | Production of transductantsb | Tested transductants
|
|
|---|---|---|---|---|---|
| No. | H antigen identified | ||||
| Section A | |||||
| RH 3535 | 200PS | H48 | Yes | 15 | H7a,c |
| B99-2 | H6 | Yes | 10 | H7a,c | |
| EJ34 | None | Yes | 16 | H7a,c | |
| 200PS | H48 + H7 | No | |||
| B99-2 | H6 + H7 | No | |||
| EJ34 | H7 | No | |||
| U5/41 | 200PS | H48 | Yes | 10 | H7a,b |
| B99-2 | H6 | Yes | 10 | H7a,b | |
| EJ34 | None | Yes | 10 | H7a,b | |
| 200PS | H48 + H7 | No | |||
| B99-2 | H6 + H7 | No | |||
| EJ34 | H7 | No | |||
| A42 | 200PS | H48 | Yes | 10 | H7a,c |
| B99-2 | H6 | Yes | 11 | H7a,c | |
| EJ34 | None | Yes | 10 | H7a,c | |
| 200PS | H48 + H7 | No | |||
| B99-2 | H6 + H7 | No | |||
| EJ34 | H7 | No | |||
| Section B | |||||
| 200PS:H7RH3535 | EJ34 | None | Yes | 29 | H7a,c |
| EJ34 | H7 | No | |||
| 200PS:H7U5/41 | EJ34 | None | Yes | 30 | H7a,b |
| EJ34 | H7 | No | |||
| 200PS:H7A42 | EJ34 | None | Yes | 30 | H7a,c |
| EJ34 | H7 | No | |||
| 200PS | EJ34 | None | Yes | 16 | H48 |
a
Strains 200PS:H7RH3535, 200PS:H7U5/41, and 200PS:H7A42 are the designations of transductant classes obtained from recipient strain 200PS with donor strains RH 3535, U5/41, and A42, respectively. Three transductants of each class were used as donors in experiments with EJ34 as the recipient; and 9 to 10 second-step transductants from each cross, if any resulted, were tested.
b
Yes, transductants resulted; no, no transductants were found.