TABLE 5.

Comparative study of flagellar antigens of transductants and donor wild strains by antibody absorption technique

Strain used as vaccine for antiserum production	Strain used for absorption	Titer of H antibodies to straina:
		U5/41	B99:H7U5/41	A42	B99:H7A42	RH 3535	B99:H7RH3535	B99-2b
U5/41	None	51,200	51,200	51,200	51,200	51,200	51,200	—
	B99:H7U5/41	—	—	—	—	—	—	NT
	B99:H7A42	1,600	1,600	—	—	—	—	NT
	B99:H7RH3535	800	1,600	—	—	—	—	NT
A42	None	25,600	25,600	25,600	25,600	25,600	25,600	—
	B99:H7U5/41	—	—	1,600	1,600	1,600	1,600	NT
	B99:H7A42	—	—	—	—	—	—	NT
	B99:H7RH3535	—	—	—	—	—	—	NT
RH 3535	None	6,400	6,400	6,400	6,400	6,400	6,400	—
	B99:H7U5/41	—	—	200	400	400	400	NT
	B99:H7A42	—	—	—	—	—	—	NT
	B99:H7RH3535	—	—	—	—	—	—	NT
B99:H7U5/41	None	25,600	25,600	25,600	25,600	25,600	25,600	—
	U5/41	—	—	—	—	—	—	NT
	A42	800	1,600	—	—	—	—	NT
	RH 3535	800	800	—	—	—	—	NT
B99:H7A42	None	25,600	25,600	25,600	25,600	25,600	25,600	—
	U5/41	—	—	3,200	1,600	3,200	3,200	NT
	A42	—	—	—	—	—	—	NT
	RH 3535	—	—	—	—	—	—	NT
B99:H7RH3535	None	12,800	12,800	12,800	12,800	12,800	12,800	—
	U5/41	—	—	800	1,600	800	800	NT
	A42	—	—	—	—	—	—	NT
	RH 3535	—	—	—	—	—	—	NT

^aReciprocal titer by the tube agglutination assay. Strains B99:H7_U5/41, B99:H7_A42, and B99:H7_RH3535 were transductants isolated from recipient B99-2, which inherited their H antigens from wild donor strains U5/41, A42, and RH 3535, respectively. The starting dilution of the antisera was 1:25. —, titer less than 1:25; NT, not tested.

^bB99-2 was the recipient O55:H6 strain.