TABLE 2.
Inhibition of WNV-reactive MAbs by blocking ELISA with sera from chickens infected with WNVa
| MAb | Optimal MAb dilution | % Inhibition of MAb binding at time between infection and serum collection (days)b:
|
|||
|---|---|---|---|---|---|
| 14 | 19 | 28 | 31 | ||
| 2B2 | 1:40 | 79.3 ± 1.8 | 69.0 ± 3.7 | 49.7 ± 5.4 | 61.0 ± 5.7 |
| 3.1112G | 1:2,000 | 85.3 ± 0.7 | 87.8 ± 0.5 | 71.6 ± 2.0 | 82.3 ± 0.3 |
| 3.67G | 1:80 | 58.6 ± 4.7 | 67.7 ± 0.3 | 49.2 ± 4.3 | 51.8 ± 2.2 |
| 3.91D | 1:40 | 47.2 ± 6.6 | NT | NT | NT |
| 6B6C-1 | 1:16,000 | 71.8 ± 2.6 | 84.4 ± 1.9 | 72.4 ± 2.6 | 79.3 ± 2.1 |
| 3H6 | 1:4 | 60.3 ± 1.8 | NT | NT | NT |
a
MAbs were tested in triplicate. All sera were diluted 10-fold.
b
Values are means±standard deviations. NT, not tested.