TABLE 3.
Inhibition of MAbs by blocking ELISA with sera from crows infected with WNV
| Sample serum no. | Crow vaccinated | % Inhibition of MAba
|
||||||
|---|---|---|---|---|---|---|---|---|
| 2B2 | 3.1112G | 3.67G | 3.91D | 6B6C-1 | 6B5A-2 | 3H6 | ||
| 1 | Yes | 98.4 ± 0.4 | 67.2 ± 6.8 | 74.4 ± 4.5 | 52.4 | 49.9 | 74.1 | 50.0 |
| 2 | Yes | 96.4 ± 0.8 | 62.3 ± 5.5 | 89.8 ± 2.0 | 75.0 | 15.0 | 11.5 | 0 |
| 3 | No | 89.3 ± 4.2 | 71.3 ± 4.7 | 52.8 ± 4.0 | 30.2 | 35.0 | 26.6 | 14.3 |
| 4 | Yes | 69.2 ± 8.5 | 70.0 ± 5.6 | 44.8 ± 5.2 | 28.4 | 38.5 | 0 | 28.7 |
| 5 | Yes | 93.0 ± 1.1 | 65.5 ± 6.2 | 77.5 ± 5.2 | 48.8 | 76.5 | 66.1 | 69.2 |
| 6 | Yes | 87.3 ± 2.9 | 85.1 ± 3.2 | 47.8 ± 6.3 | 19.6 | 28.0 | 16.1 | 19.3 |
| 7 | Yes | 69.1 ± 7.5 | 56.0 ± 5.2 | 40.1 ± 10.8 | 39.1 | 56.0 | 0 | 40.5 |
| 8 | No | 93.9 ± 3.2 | 78.1 ± 5.7 | 57.1 ± 8.0 | 33.8 | 0 | 12.8 | 0 |
| Mean | 87.1 | 69.4 | 60.5 | 40.9 | 37.4 | 25.9 | 27.8 | |
a
Values are means±standard deviations. Inhibition values of ≥30% are considered significant. MAbs 2B2, 3.1112G, and 3.67G were tested in triplicate. All other MAbs were tested in duplicate. All sera were diluted 10-fold.