TABLE 5.
Inhibition of MAbs by blocking ELISA with anti-WNV, anti-SLEV, and normal sera from multiple avian speciesa
| Serum type and avian species | WNV PRNT90 titer | SLEV PRNT90 titer | % Inhibition of MAb:
|
||||
|---|---|---|---|---|---|---|---|
| 2B2 | 3.1112G | 3.67G | 6B6C-1 | 6B5A-2 | |||
| Anti-WNV | |||||||
| American robin | 40 | 84.8 | 86.4 | 45.8 | 24.4 | 0 | |
| Blue jay | ≥320 | 56.1 | 70.0 | 33.4 | 58.1 | 0 | |
| Canada goose-1 | ≥320 | 59.7 | 65.0 | 44.1 | 63.2 | 22.0 | |
| Canada goose-2 | ≥320 | 65.1 | 67.4 | 40.1 | 70.3 | 2.2 | |
| Japanese quail-1 | 80 | 51.0 | 60.8 | 18.1 | 65.1 | 40.7 | |
| Japanese quail-2 | 40 | 51.6 | 38.5 | 35.0 | 64.1 | 12.3 | |
| Killdeer-1 | ≥320 | 85.1 | 76.8 | 36.8 | 77.0 | 0 | |
| Killdeer-2 | 160 | 44.5 | 57.4 | 35.1 | 68.1 | 28.4 | |
| Monk parakeet | 80 | 43.3 | 56.5 | 27.9 | 68.4 | 0 | |
| Mourning dove | 40 | 41.6 | 88.6 | 2.7 | 62.4 | 0 | |
| Ring-necked pheasant-1 | 80 | 46.5 | 62.0 | 35.7 | 77.6 | 0 | |
| Ring-necked pheasant-2 | 160 | 66.0 | 72.6 | 40.5 | 80.5 | 0 | |
| Ring-necked pheasant-3 | 40 | 79.4 | 62.9 | 44.8 | 60.5 | 2.2 | |
| Mean | 59.6 | 66.5 | 33.8 | 64.6 | 8.3 | ||
| Anti-SLEV | |||||||
| Domestic turkey | 640 | 49.3 | 17.7 | 16.8 | 91.4 | 94.1 | |
| European starling-1 | ≥40 | 20.2 | 6.3 | 7.2 | 75.4 | 47.4 | |
| European starling-2 | 20 | 45.4 | 0.7 | 7.7 | 64.9 | 93.9 | |
| Graylag goose | 160 | 44.5 | 13.1 | 15.2 | 91.9 | 83.3 | |
| House sparrow | 160 | 57.0 | 7.5 | 18.0 | 93.2 | 85.0 | |
| Rock dove-1 | 320 | 86.0 | 0 | 15.4 | 76.8 | 87.7 | |
| Rock dove-2 | 40 | 12.2 | 0 | 9.3 | 65.1 | 11.8 | |
| Mean | 44.9 | 6.5 | 12.8 | 79.8 | 71.9 | ||
| Normal | |||||||
| American crow-1 | 9.6 | 15.7 | 13.8 | 0 | 15.0 | ||
| American crow-2 | 5.5 | 11.1 | 5.6 | 0 | 7.0 | ||
| American crow-3 | 4.2 | 5.0 | 0 | 0 | 0 | ||
| American robin-1 | 0 | 8.2 | 0.6 | 0 | 14.6 | ||
| American robin-2 | 0 | 9.5 | 0 | 0 | 5.3 | ||
| Graylag goose | 10.1 | 5.0 | 5.5 | 21.9 | 0.6 | ||
| House finch | 15.2 | 0 | 0 | 4.1 | 0 | ||
| House sparrow | 3.6 | 10.8 | 2.2 | 12.7 | 7.2 | ||
| Japanese quail | 11.6 | 5.6 | 3.2 | 0 | 0 | ||
| Monk parakeet | 0 | 0 | 1.6 | 0 | 0 | ||
| Mourning dove | 4.5 | 2.0 | 5.7 | 0 | 0 | ||
| Northern bobwhite | 15.8 | 5.8 | 3.7 | 24.3 | 0 | ||
| Mean | 6.7 | 7.0 | 3.5 | 5.2 | 4.1 | ||
a
Titers of <10 are not shown. Inhibition values of ≥30% are considered significant. All sera were diluted 10-fold.