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. 2003 Mar;41(3):998–1003. doi: 10.1128/JCM.41.3.998-1003.2003

FIG. 1.

FIG. 1.

Electrophoretic gel profile of RT-PCR products generated using the Visible Genetics TRUGENE HIV-1 genotyping test. One HIV-1 isolate was selected from each HIV-1 subtype for analysis using the TRUGENE kit (version 1.0) and prototype 1.5 RT-PCR primers. The 1.5 RT-PCR primers were substituted for the kit RT-PCR primers and cycling parameters were modified. Lanes 1 and 12 contain 100-bp DNA ladders. Lanes 2 through 9 contain subtypes A, B, C, D, E, G, F, and H. Lane 10 contains the positive control from the kit. Lane 11 contains a negative specimen control. (A) Gel profile of amplified product generated by the TRUGENE kit (version 1.0). (B) Gel profile of amplified product generated using the prototype 1.5 RT-PCR primers with the TRUGENE kit.