FIG. 2.

PCR fragments obtained with primer pair A1C-Lok1 that covers the region from the gene tcdA to the 3′ end of PaLoc. Fragments were amplified in three A⁻B⁺ strains (8864, SUC 36, and J9965) and compared to the reference strain VPI 10463. Strains VPI 10463 and 8864 were controls representing a full-length PaLoc and 3′-deleted form of PaLoc, respectively. Strain SUC 36, a new representative of A⁻B⁺ strains, has only a small deletion in this region. In another new type of A⁻B⁺ strain (J9965), the deletion is larger but ∼2 kb more are present than in C. difficile 8864.