TABLE 1.
Cost determination of individual tests used in conventional identification of NTM, based on the simultaneous testing of four isolates and one set of test controlsa
Biochemical test | Cost (CAD$) | Slow growers
|
Rapid growers | |
---|---|---|---|---|
Inert | Active | |||
Arylsulfatase (3 day) | 5.35 | √ | √ | √ |
Arylsulfatase (14 day) | 5.35 | √ | √ | √ |
Tween (5 day) | 3.17 | √ | √ | √ |
Urease | 3.17 | √ | √ | √ |
Nitrate | 4.81 | √ | √ | √ |
68°C catalase | 4.49 | √ | √ | √ |
Semiquantitative Catalase | 5.70 | √ | √ | √ |
Iron uptake | 4.88 | √ | - | √ |
NaCl | 4.89 | √ | - | √ |
Growth on MAC | 4.58 | √ | - | √ |
Citrate | 4.43 | √ | - | √ |
Tellurite reduction | 6.04 | √ | √ | √ |
Pyrazinamidase (4 day) | 5.15 | √ | √ | √ |
Pyrazinamidase (7 day) | 5.15 | √ | √ | √ |
Beta-glucosidase | 4.44 | √ | - | - |
Acid phosphatase | 5.29 | √ | - | - |
Amidases | ||||
Nicotinamidase | 7.35 | √ | - | - |
Acetamidase | 4.71 | √ | - | - |
Allantoinase | 7.35 | √ | - | - |
Benzamidase | 7.35 | √ | - | - |
Succinamidase | 7.35 | √ | - | - |
Carbohydrates | ||||
Fructose | 4.71 | √ | - | √ |
Mannitol | 4.71 | √ | - | √ |
Inositol | 4.71 | √ | - | √ |
Sorbitol | 4.71 | √ | - | √ |
Temperatures (°C) | ||||
25 | 3.54 | √ | √ | √ |
28 | 3.54 | √ | √ | √ |
31 | 3.54 | √ | √ | √ |
37 | 3.54 | √ | √ | √ |
42 | 3.54 | √ | √ | √ |
Pigment production | ||||
Light at 37°C | 3.54 | √ | √ | √ |
Dark at 37°C | 3.54 | √ | √ | √ |
Purchased tests: | ||||
Niacin | 7.80 | √ | √ | √ |
Polymyxin B | 5.43 | √ | - | √ |
Ciprofloxacin | 5.43 | √ | - | √ |
Total (CAD$) | 173.23 | 80.93 | 129.40 |
Tests performed within a particular group of organism are marked with a check.