Figure 3.

GFP-Bud6 localization in bud3 haploids. (A) Selected frames from a 78-min time-lapse series showing the initial localization of GFP-Bud6 at the bud tip followed by faint label of the neck (arrowhead) in bud3 cells (MYC4BG). The bulk of the label continued to associate with the distal bud surface as was the case in wild-type diploids. Numbers indicate time in minutes relative to bud emergence for the cell on the left. (B) Plot showing linescans through the polarity axis at 30 min after bud emergence in a bud3 haploid cell. For reference, the labeling pattern of a wild-type haploid shown in Figure 2C has been overlaid (gray line).