Figure 6.

bud6Δ phenotypes at later stages of the spindle pathway. (A) Exaggerated spindle oscillations across the bud neck in a bud6 haploid cell (MYC2T). In spite of correct alignment along the mother-bud axis, transits across the bud neck continued for at least 70 min after completion of spindle assembly. At the same time, spindle elongation paused at a length of 3.2 μm. (B) Cytoplasmic microtubule behavior of bud6Δ cells (MYC2T) in late anaphase. Microtubules emanating from the SPB_daughter extend across the neck and into the mother cell. Late anaphase spindles are excessively long and tend to curve. Bars, 2 μm.