Figure 2.

Rac signaling pathways contribute to the destabilization of cadherin receptors after Ras activation. HaCat cells were microinjected with an expression vector containing dominant-negative Rac (N17Rac, a–c), oncogenic Ras (V12Ras, d–f), or both together (g–i). After 5 h, cells were fixed and double labeled for the myc tag (b, e, and h) and E-cadherin (c, f, and i). Injected patches were identified by coinjection of Dextran-Texas Red (a, d, and g). Arrows (c and i) point to the presence of cadherin staining at junctions; arrowhead (f) shows the absence of cadherin staining between two expressing cells. Bar, 50 μm.