Figure 2.

Pep12p with the Sso1p TMD (PSSO) reaches the outer vacuolar membrane via late endosomes. (A) Wild-type cells (wt) or indicated mutants expressing GFP–Pep12p or PSSO. GFP–Pep12p labels vacuoles, as shown by the FM4-64 staining; arrowheads indicate equivalent positions in these double-label images. PSSO labels vacuoles in wild-type and Δapm3 cells but labels perivacuolar PVC in vps4 cells, which also labels with FM4-64 (arrowheads). (B) Deletion mutants of PSSO, lacking the indicated residues of Pep12p sequence, are also found on the outer vacuolar membrane. The junction with Sso1p occurs at residue 261.