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. 2000 Nov;11(11):3737–3749. doi: 10.1091/mbc.11.11.3737

Figure 6.

Figure 6

Proteolytic processing of chimeras in the vacuole. (A) GFP-tagged Snc1p with the Nyv1p TMD (GSN; apparent size 45 kDa) and GFP–Nyv1p (NYV; apparent size 60 kDa) were expressed in wild-type (WT) or pep4 cells and detected by immunoblotting with anti-GFP. The position of free GFP generated by proteolysis of GSN is indicated. (B) pho8 pho13 cells were transformed with vector alone (pRS416) or with plasmids expressing ALP–Pep12p–Sso1p chimeras without (APSSO416) or with (APSSO3D416) an aspartic acid at position 3 of the TMD. ALP activity was assayed in cell lysates.