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Effects of TMD mutations on Sso1p location. Confocal images are shown of cells expressing GFP-tagged Sso1p with the indicated representative mutations (D or R, at positions indicated by numbers). The distribution of each construct is shown in wild-type (wt) and end4 cells. Note that 3D and 5D are the only mutant proteins that are not fully delivered to the plasma membrane in end4 cells.
