Fig. 5. Disruption of the PfRh1 gene in 3D7. (A) Pr represents the probe used for Southern hybridization in (B). The restriction enzyme shown as N is NsiI. pHTkΔrh1 has integrated into PfRh1 by a double recombination crossover event to yield 3D7ΔRh1. (B) Southern hybridization of genomic DNA digested with NsiI from 3D7, 3D7ΔRh1/1 and 3D7ΔRh1/2. Sizes on the right are in kb. (C) Western blot of supernatants from W2mef, 3D7, 3D7ΔRh1/1, 3D7ΔRh1/2 with antibodies to PfRh1 (first panel) and EBA175 (second panel).