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Western blot analysis of NTR protein expression under aerobic and hypoxic conditions. Protein lysates from wild-type HT1080, CMV/IE-NTR, and 5HRE/hCMVmp-NTR cells after treatment under aerobic or hypoxic conditions (0.02% O2) for 6 to 18 hours were analyzed by SDS-PAGE and immunoblotting with a monoclonal antimyc antibody as described in Materials and Methods section. A duplicate membrane was also treated for an anti-β-actin antibody as a loading control.
