Figure 2.

Experimental and theoretical biodistribution profiles of sensitized (A) and normal (B) lymphocyte populations in tumor-bearing mice and in tumor-bearing mice with splenectomy (C). Overall patterns of systemic distribution and accumulation of lymphocytes within the tumors were similar for both populations of lymphocytes, suggesting that the initial phase (<7 days) of cell recruitment to tumors may be nonspecific. Splenectomy of mice before the implantation of the tumor results in tumor accumulation kinetics comparable to the nonsensitized lymphocyte populations in the nonsplenectomized mice. Retention of transferred lymphocytes within the liver is greater than in nonsplenectomized mice, suggesting a shift in compartmental distribution following the removal of a major filtration compartment, but only a slight increase in tumor localization can be observed. Means ±SD are shown, n=6 mice/group. The simulations of the mathematical model are shown overlying the experimental data. The predictions agree well with the experiments and capture the salient features of the experimental biodistribution profiles.