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. 2003 Mar 3;22(5):1075–1087. doi: 10.1093/emboj/cdg100

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Copyright © 2003 European Molecular Biology Organization

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graphic file with name cdg100f4a.jpg — Fig. 4. Kinase activity of Bub1p is required but not sufficient for spindle checkpoint. (A) Conservation of K762 residue in the kinase domain of Bub1p. (B) Left panel: kinase mutants. bub1-K762R carries a point mutation and bub1-Δkinase lacks the entire kinase domain. Right panel: cell extracts from exponentially growing bub1-HA, bub1-K762R-HA and bub1-Δkinase-HA strains were analysed by western blotting using anti-HA antibody. Cdc2p is shown as loading control. (C) See Figure 33 legend for details. The concentrations of TBZ used are indicated in µg/ml. (D) Cdc2-M26 (WT), cdc2-M26 bub1-K762R (bub1-K762R), cdc2-M26 bub1Δkinase (bub1Δkinase), cdc2-M26 bub1-P*-Δkinase (bub1-P*-Δkinase) and cdc2-M26 bub1Δ (bub1Δ) strains were used for the experiment described in Figure 33B. (E) The experiment described in Figure 33C was performed using kinase mutants.

graphic file with name cdg100f4b.jpg — Fig. 4. Kinase activity of Bub1p is required but not sufficient for spindle checkpoint. (A) Conservation of K762 residue in the kinase domain of Bub1p. (B) Left panel: kinase mutants. bub1-K762R carries a point mutation and bub1-Δkinase lacks the entire kinase domain. Right panel: cell extracts from exponentially growing bub1-HA, bub1-K762R-HA and bub1-Δkinase-HA strains were analysed by western blotting using anti-HA antibody. Cdc2p is shown as loading control. (C) See Figure 33 legend for details. The concentrations of TBZ used are indicated in µg/ml. (D) Cdc2-M26 (WT), cdc2-M26 bub1-K762R (bub1-K762R), cdc2-M26 bub1Δkinase (bub1Δkinase), cdc2-M26 bub1-P*-Δkinase (bub1-P*-Δkinase) and cdc2-M26 bub1Δ (bub1Δ) strains were used for the experiment described in Figure 33B. (E) The experiment described in Figure 33C was performed using kinase mutants.