Fig. 7. Bub1p kinase activity is required for maintenance of centromeric Rec8p during anaphase I and reductional segregation. (A) Spore viability (%) and equational segregation frequency (%) of WT, bub1Δ, bub1-P*, bub1-Δkinase, bub1-K762R and mad2Δ cells. (B) In vivo localization of Bub1–GFPp (WT), Bub1-P*–GFPp (bub1-P*), Bub1-Δkinase–GFPp (bub1-Δkinase) and Bub1-K762R–GFPp (bub1-K762R) during meiosis. (C) Rec8–GFPp localization during anaphase I in WT, bub1Δ, bub1-P*, bub1-Δkinase and bub1-K762R cells.