Fig. 2. Potentiation of GR signaling by the immunophilins. (A) Reporter expression rates of the GR reporter cells transformed with an empty vector or with plasmids expressing FKBP52, FKBP51, CPR7, CyP40 or PP5. Each bar is the average expression rate (±SD) from quadruplicate cultures of a representative isolate. (B) Steady state levels of GR in the parental strain W303a and GR reporter strains in FKBP51, FKBP52 or control vector backgrounds. Extracts from each strain were analyzed by western blotting for GR (upper panel) and for the ribosomal protein L3 as a loading control (lower panel). (C) Similar to (A) except that GR reporter cells were transformed with a single vector or two vectors (52+52, 51+52 or 52+PP5) for co-expression of immunophilins. (D) For each strain shown in (C), the total cell extract was immunostained for the proteins indicated.