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Rpl11b–GFP is incorporated in the 60S ribosomal subunit. Yeast lysate from wild-type cells expressing Rpl11b–GFP from a CEN plasmid was separated on a 7–30% sucrose gradient. The sucrose gradient profile was recorded by absorbance at 254 nm (upper panel). Fractions 8–19 collected from the sucrose gradient and lysate (5 μg of total protein) were probed with anti-GFP to detect Rpl11b–GFP (bottom panel).
