Figure 3.

Rpl11b–GFP localization assay. Wild-type (PSY580) or mtr4-1 cells expressing Rpl11b–GFP from a CEN plasmid were grown to early log phase, shifted to 37°C for 1 h, and then shifted back to 25°C for up to 1 h. Yeast cells were fixed and incubated with DAPI to stain the nuclei. Rpl11b–GFP was visualized by fluorescence microscopy. Panels a, b, e, and f show cells shifted to 37°C for 1 h, and panels c, d, g, and h show cells shifted back to 25°C for 30 min.