Figure 6.

Deletion of KAP120 causes a large reduction in 60S ribosomal subunit levels. Yeast lysates prepared from KAP120 and kap120Δ cells expressing Rpl11b–GFP from a CEN plasmid were centrifuged through 7–30% sucrose gradients, and gradient profiles were collected by absorbance at 254 nm. An overlay of the gradient profiles for KAP120 and kap120Δ cells is shown to emphasize the difference in peak heights for the 60S and 40S ribosomal subunits. Equal amounts of total RNA (200 μg) were loaded for each gradient.