Figure 2.

Effects of the DRz4 enzyme on gene expression and of apoptosis. After 40 hours transfection time, cytoplasmic protein extracts were prepared from control (DOTAP) and DRz4- and DRz4r-treated cells. Fifteen micrograms from each sample was analyzed by Western blots using specific antibodies (A). The final concentrations of the DRz4 and DRz4r are indicated. A potential cross-reactive protein is indicated by the open arrow. A higher exposure of the data is shown in B. (C) Quantification of cellular DNA fragmentation by the TUNEL method. Controls and DRz4-treated cells were analyzed by the TUNEL method as described in Materials and Methods section. Of the DNA enzyme-treated cells, 82% were apoptotic. Cells were transfected for 48 hours.