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. 2002 Nov;4(6):551–557. doi: 10.1038/sj.neo.7900270

Figure 2.

Figure 2

Timing of BID, procaspase 3, and PARP cleavage in SW480 and HCT116 cells after TRAIL treatment. (A) The cells were treated with TRAIL in the presence of the different inhibitors for the indicated times — 4, 8, and 24 hours. The molecular composition of the different caspase inhibitors is given in Materials and Methods. BID cleavage was analyzed through Western analysis. (B) The cleavages of procaspase 3 and PARP were tested after 4, 8, and 24 hours of TRAIL treatment. Different caspase inhibitors were used to show the difference in their effects on procaspase 3 and PARP cleavage in HCT116 and SW480 cells. Caspases 9 and 2 inhibitors prevent procaspase 3 and PARP cleavages in HCT116 cells (see 8-hour time point). “M” stands for mock, and “T” or “Tr” for TRAIL treatment.