Figure 9.

Blot overlay analysis of the interaction between 4.1R-10 kDa domain and F-actin, HMM, and tropomyosin. (A) Similar amounts of GST-10 kDa fusion proteins that either carry or omit exon 16 as well as GST protein alone were subjected to SDS-PAGE and subsequently stained with Coomassie blue. Blots identical to the one shown in A were incubated with purified skeletal muscle F-actin (B), HMM (C), and tropomyosin (D) followed by Western blotting with anti-actin JLA20 mAb (B), anti-myosin MF20 mAb (C), and anti-tropomyosin CH1 mAb (D).