Figure 9.

Rab6A′ Q72L A87T interacts with Rabkinesin-6 and redistributes Golgi proteins into the ER. (A) The S. cerevisiae reporter strain L40 was cotransformed with pLexA–Rab6A Q72L, pLexA–Rab6A′ Q72L, or pLexA–Rab6A′ Q72L A87T and pGADGH–Rabkinesin-6. Transformants were analyzed as described in the legend to Figure 8. (B) HeLa cells transfected with Rab6A′ Q72L A87T–encoding plasmid were fixed 5 h after transfection with 4% paraformaldehyde. Cells were double labeled with a mAb to the medial Golgi antigen CTR433 (middle panel, green staining) and anti-Rab6 polyclonal antibody (left panel, red staining). The right panel shows the superimposition of the two labeling patterns. Asterisks indicate the cells that highly overexpressed Rab6A′ Q72L A87T mutant.