Fig. 1.

The effect of DNA dosage in IFN transgene treatment of MCMV infection and myocarditis. Bupivacaine-treated BALB/c mice were inoculated with doses of either 50μg or 100μg of Blank, IFNA6 or IFNB DNA at 14 days prior to viral challenge with 1x104 pfu of MCMV i.p. Viral titres were determined by plaque assay in (A) spleen and (B) liver at day 3 p.i. and (C) salivary glands at day 7 p.i. Results are expressed as mean pfu/g ± SEM (n=5). (D) Myocarditis was evaluated at day 7 p.i. and is expressed as the number of foci per heart section ± SEM (n=5). *, Statistical significance (p≤0.05) compared with Blank treatment groups.