Figure 3.

Src mediates CSF-1–induced cyclin D2, but not D1 or c-myc expression. BMMs were isolated from the wild-type, Src^+/−, and Src^−/− mice. The 11th-d cultures were G1 arrested in CSF-1–free media and unstimulated or stimulated with CSF-1 (4.4 nM) for 4 h. Total RNA was isolated and CSF-1–stimulated D2 (A), D1 (B), c-myc (C), and GAPDH (D) gene expression was analyzed by Northern blot analysis (15 μg/lane). The relative fold increases (measured by scanning densitometry) were based on calibration with the corresponding GAPDH levels: band⁺/band⁻ X GAPDH⁻/GAPDH⁺ = Fold.