Figure 4.

Establishment and growth curves of v-Src and myc-ER stable cell lines. BAC1.2F5 cells stably expressing v-Src or myc-ER were established by retrovival infection as described in MATERIALS AND METHODS. Equal numbers of the parental BAC1.2F5, 2F5-pMV7 (vector), 2F5-v-Src, and 2F5-myc-ER (clone 4) cells were seeded in duplicates, and proliferation under various conditions was compared for 6 d by counting the cells each day in triplicates and plotting the means (the SDs ranged from 4 to 8%). Results of one representative experiment are shown.