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. 2000 Nov;11(11):3835–3848. doi: 10.1091/mbc.11.11.3835

Figure 7.

Figure 7

Roles for the MEK/ERK pathway in CSF-1–induced cyclin D2 gene expression. BAC1.2F5 cells were pretreated with or without indicated concentrations of MEK1 inhibitor PD98059 (15 min) before CSF-1 stimulation (13.2 nM, 5 min for A and F, and 5 h for B to E), and were subjected to either Western blot with antiactive ERK antibodies (A and F) or Northern blot analysis (B–E). (A) Effect of PD98059 on CSF-1–stimulated ERK1 and ERK2 activation. (B) Effect of PD98059 on D2 expression. (C) Effect of PD98059 on D1 expression. (D) Effect of PD98059 on c-myc expression. (E) GAPDH control. (F) CSF-1–stimulated activation ERK1 and ERK2 in cells expressing the four D2 expression-affected CSF-1R mutants. Induction was measured by scanning densitometry and was given in fold increase after calibration with corresponding GAPDH levels of each cell line: band+/band− × GAPDH−/GAPDH+ = Fold. “+” and “−” represent “with” and “without” CSF-1 stimulation.