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. 2000 Nov;11(11):3849–3858. doi: 10.1091/mbc.11.11.3849

Figure 4.

Figure 4

The SSO1 Q-to-R mutant displays a conditional growth defect that is suppressed by the expression of SNC2 with an R-to-Q substitution. (A) Each of the plasmids shown was introduced into SSO1/2 disruption strain (a; SSO1Δ::LEU2; SSO2Δ::HIS3; ade2-1; his3-11,15; ura3-1; trp1-1, pGAL-SSO1); transformants were selected on medium containing galactose and tested for viability on 2% glucose (YPD) and 3% galactose (YP-Gal) at the indicated temperatures. (B) SNC2 or SNC2-R52Q plasmids (CEN-TRP1) were introduced into the yeast strains containing sso1-Q224R (CEN-URA3) as the only source of Sso1 protein (generated as described in A). Transformants were selected on glucose medium lacking tryptophan and uracil and tested for viability at the permissive and restrictive temperatures.